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What is the role of alkaline copper reagent?

What is the role of alkaline copper reagent?

The role of alkaline copper reagent is : Reduction of cupric ions to cuprous ions.

What is alkaline copper solution?

The copper plating is applied exclusively by electroplating, done in electrolytic baths in which the object to be coppered acts as a cathode, while the anode is made of pure copper cathodes. In alkaline baths the electrolyte is a solution of the complex cyanide Na₃Cu (CN) 4.

What are the components of alkaline copper reagent?

Alkaline copper reagent: Solution A: 2% sodium carbonate in 0.1 N sodium hydroxide. Solution B: 0.5% copper sulphate in 1% sodium potassium tartarate.

Which reagent used in estimation of sugar by Folin WU method?

Alkaline copper reagent
Estimation of Blood Sugar by Folin-Wu Method15

Folin-wu tubes Distilled water Alkaline copper reagent
B 2 ml 2 ml
S 2 ml
T 2 ml

How do you make an alkaline copper reagent?

Reagents. Dissolve 20 gm sodium carbonate in 260 ml water, 0.4 gm cupric sulfate (5x hydrated) in 20 ml water, and 0.2 gm sodium potassium tartrate in 20 ml water. Mix all three solutions to prepare the copper reagent.

Which product reacts with Folin Ciocalteu reagent?

Folin and Ciocalteu noted during the development of the assay that tryptophan, which is non-phenolic, is reactive towards the reagent and contributes towards protein absorbance in the Folin-Lowry Assay (1).

How do you make an alkaline copper solution?

Reagents. Dissolve 20 gm sodium carbonate in 260 ml water, 0.4 gm cupric sulfate (5x hydrated) in 20 ml water, and 0.2 gm sodium potassium tartrate in 20 ml water. Mix all three solutions to prepare the copper reagent. Prepare 100 ml of a 1% solution (1 gm/100 ml) of sodium dodecyl sulfate (SDS).

What is Folin Wu method?

Principle. Glucose reduces the cupric ions present in the alkaline copper reagent to cuprous ions or the cupric sulfate is converted into cuprous oxide, which reduces the phosphomolybdic acid to phosphomolybdous acid, which is blue when optical density is measured at 420 nm.

What is O-toluidine method?

Abstract. o-Toluidine, 6% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3% (w/v) trichloracetic acid. A stable green color develops after heating at 100° for 10 min., and the absorbance is determined at 630 or 635 mµ.

How reagent A is prepared in Folin method?

The phenolic compounds react with the Folin reagent which is formed by sodium tungstate and sodium molybdate in acid medium. Thus, this reaction produces a yellow complex that can be reduced with the phenolic compounds and identified with a spectrophotometric method at a wavelength of 765 nm (Cueva et al., 2010).

How to reduce sugar with Alkaline copper-citrate reagent?

These requirements are met by a method described by Scales, in which the cuprous o}”ide formed by the reduction of alkaline copper-citrate reagent is titrated with iodine [3, 4]. According to the original method the sugar solution (10 ml) is mixed with 20 ml of the copper reagent, brought to boiling in 4 minutes, and boiled for 3 minutes.

What kind of reagent is used to precipitation copper?

The use of some gas or reagent by which the copper is obtained, usually in the form of an intermediate precipitate which requires further treatment. For example, the precipitation of the copper as Cu2Cl2 from chloride solutions; with SO2 gas. The use of iron is adapted to almost any process.

How long does it take to boil copper reagent?

According to the original method the sugar solution (10 ml) is mixed with 20 ml of the copper reagent, brought to boiling in 4 minutes, and boiled for 3 minutes. After cooling the solution, aqueous acetic acid, an excess of I jligures in brackets indicate the literature references at the end of this paper. 241

How much copper sulfate is in Benedict’s reagent?

One litre of Benedict’s reagent can be prepared by mixing 17.3 grams of copper sulfate pentahydrate (CuSO 4.5H 2 O), 100 grams of sodium carbonate (Na 2 CO 3), and 173 grams of sodium citrate in distilled water (required quantity).